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hypervariable region v3–v4 of the 16 s rrna gene  (Macrogen)

 
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    Macrogen hypervariable region v3–v4 of the 16 s rrna gene
    Hypervariable Region V3–V4 Of The 16 S Rrna Gene, supplied by Macrogen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hypervariable+region+v3%E2%80%93v4+of+the+16+s+rrna+gene/amplicon+sequencing+of+the+v3+v4+regions+of+the+16s+rrna+gene/pm40610442-94-9-16
    Average 90 stars, based on 1 article reviews
    hypervariable region v3–v4 of the 16 s rrna gene - by Bioz Stars, 2026-08
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    Sampling protocol and analysis method for participants. The subjects were grouped according to gestational age (37 weeks). Fecal samples were collected at 7 and 28 days after birth. ONT was performed on 38 fecal samples obtained from 34 infants. To compare the sequencing results, 15 overlapping samples from 13 infants were analyzed using Illumina. T, Term infants; P, Preterm infants; 7D, day 7 of life after birth; 28D, day 28 of life; DNA, Deoxyribonucleic Acid; rRNA, Ribosomal ribonucleic acid; ONT, Oxford Nanopore Technologies.

    Journal: Frontiers in Microbiology

    Article Title: Gut microbiome profiling of neonates using Nanopore MinION and Illumina MiSeq sequencing

    doi: 10.3389/fmicb.2023.1148466

    Figure Lengend Snippet: Sampling protocol and analysis method for participants. The subjects were grouped according to gestational age (37 weeks). Fecal samples were collected at 7 and 28 days after birth. ONT was performed on 38 fecal samples obtained from 34 infants. To compare the sequencing results, 15 overlapping samples from 13 infants were analyzed using Illumina. T, Term infants; P, Preterm infants; 7D, day 7 of life after birth; 28D, day 28 of life; DNA, Deoxyribonucleic Acid; rRNA, Ribosomal ribonucleic acid; ONT, Oxford Nanopore Technologies.

    Article Snippet: While Illumina sequences the V3–V4 hypervariable regions of the 16 s rRNA gene ( ) and yields >99% read accuracy using the sequencing-by-synthesis method, ONT sequences the whole hypervariable regions of 16S rRNA genes from V1 to V9 using the nanopore (pore-forming protein) method and yields around 91–92% read accuracy (R9.4.1 nanopore).

    Techniques: Sampling, Sequencing